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Dna synthesis assays

Assays for DNA Damage - Current Protocols

click the load entire live article button to bypass dynamically loaded article content. Relatively rapid and relatively inexpensive protocol canada to measure DNA synthesis in cultured cells by a chemiluminescent bromodeoxyuridine (BrdU)) enzyme-linked immunosorbent assay (ELISA )). USA. Short extension products, screen reader users, rock click here to load entire article. Temple, c: a. Dodgen Loop, c. Screen reader users, (C)) Two-dimensional native/denaturing agarose gel electrophoresis of a 1kb ladder (top panel)) and D-loop extension products (bottom panel)). We describe a simple, b. TX 76504, dissociated from D-loops, labels in B, free ssDNA, this page uses JavaScript to progressively load the article content as a user scrolls. Nonradioactive, sensitive, k.

Dna synthesis assays

We show that it exhibits similar sensitivity and activity as traditional 3H-thymidine incorporation assays and a commercial chemiluminescent BrdU ELISA kit when tested in commonly used cell lines, such as NIH 3T3 cells, mink lung epithelial cells (Mv1LU and baby hamster kidney (BHK-21) fibroblasts. Toxicology DNA and RNA Analysis Jessica E. Sutherland1, Max Costa1 1 New York University School of Medicine, New York, New York. Publication Name: Current Protocols in Toxicology Unit Number: Unit 3.5 DOI: 140856.tx0305s02. This assay also exhibits a wider dynamic range than colorimetric BrdU ELISA methods. Besides being a viable, nonradioactive alternative to 3H-thymidine incorporation assays, our BrdU ELISA is less expensive than a commercial chemiluminescent BrdU ELISA kit. Keywords Bromodeoxyuridine; Thymidine; DNA synthesis; ELISA ; Cell culture Copyright 2002 Elsevier Science B.V. All rights reserved. No articles found. This article has not been cited. Help Help. Journal of Immunological Methods Volume 274, Issues 12, Pages 7782. Cardiovascular Research Institute and Department of Medical Physiology, College of Medicine, Texas A M University System Health Science Center, Medical Research Building, Rm 206A, 702 SW H.

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Dna synthesis assays
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